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Hepatocyte Isolation After Laparoscopic Liver Resection.

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Primary human hepatocytes can be isolated from liver tissue obtained via laparoscopic liver resections (LLRs). While overall cell yield is lower from LLRs compared to open liver resections (OLRs), tissue from left-lateral LLRs provides comparable results to OLRs.

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Area of Science:

  • Hepatology
  • Surgical Innovation
  • Cell Biology

Background:

  • Primary human hepatocytes are crucial for research and drug development.
  • Traditionally, liver tissue for hepatocyte isolation is sourced from open liver resections (OLRs).
  • Laparoscopic liver resections (LLRs) are increasingly used, but their suitability for hepatocyte isolation is unknown.

Purpose of the Study:

  • To evaluate the feasibility and outcome of primary human hepatocyte isolation from laparoscopically resected liver tissue.
  • To compare cell yield, viability, and function of hepatocytes isolated from OLRs versus LLRs.

Main Methods:

  • A total of 22 hepatocyte isolations were performed using a two-step collagenase perfusion technique.
  • Liver tissue was obtained from 15 OLRs and 7 LLRs.
  • Isolation parameters (cell yield, viability, Percoll survival) and hepatocyte function (plating efficiency, urea, albumin, AST) were assessed.

Main Results:

  • Significantly higher total cell yield (36.81 × 10^6 cells/g) and viable yield (31.70 × 10^6 cells/g) were observed in the OLR group compared to the LLR group (16.84 × 10^6 and 14.70 × 10^6 cells/g, respectively).
  • Subgroup analysis indicated that right-lateral LLRs yielded poorer results, while left-lateral LLRs yielded comparable results to OLRs.
  • Hepatocyte function assays showed no significant differences between hepatocytes from OLRs and left-lateral LLRs.

Conclusions:

  • Hepatocyte isolation from laparoscopically resected liver tissue is feasible but yields lower cell counts overall compared to open surgery.
  • Liver tissue from left-lateral laparoscopic liver resections is an excellent source for primary human hepatocytes, comparable to open resections.
  • This study provides the first data on hepatocyte isolation from LLRs, highlighting the potential of minimally invasive approaches for sourcing hepatocytes.