Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PRMT5 inhibition disrupts detained intron splicing and impairs ATR signaling with increased DNA damage.

Communications biology·2026
Same author

Activity-dependent lipid droplet biogenesis and turnover regulate synaptic integrity.

bioRxiv : the preprint server for biology·2026
Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026
Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same author

Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models.

The Journal of clinical investigation·2026
Same author

A human lysosomal storage disorder toolkit for decoding proteome landscapes in cortical-like and dopaminergic-like induced neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 16, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.4K

Sample preparation for proteomic analysis using a GeLC-MS/MS strategy.

Joao A Paulo1

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Journal of Biological Methods
|August 3, 2016
PubMed
Summary

In-gel digestion coupled with mass spectrometry (GeLC-MS/MS) offers a versatile method for protein identification. This technique combines gel electrophoresis and mass spectrometry for comprehensive proteomic analysis.

Keywords:
GeLC-MS/MSbiomarkersmass spectrometryproteomics

More Related Videos

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11:32

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Published on: February 22, 2019

12.0K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.7K

Related Experiment Videos

Last Updated: Feb 16, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.4K
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11:32

Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels

Published on: February 22, 2019

12.0K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

36.7K

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Protein identification and characterization are crucial in biological research.
  • Classical and modern biochemical strategies are often combined for advanced analyses.

Purpose of the Study:

  • To review the in-gel digestion coupled with mass spectrometric analysis (GeLC-MS/MS) approach.
  • To highlight its versatility for targeted and proteome-wide analyses.

Main Methods:

  • Proteins are reduced, alkylated, and precipitated.
  • Fractionation is performed using SDS-PAGE, followed by in-gel digestion.
  • Peptides are extracted, desalted, and analyzed via LC-MS/MS.

Main Results:

  • GeLC-MS/MS integrates SDS-PAGE visualization with mass spectrometry capabilities.
  • It allows for visible assessment of protein amount and quality.
  • Compatible with various sample types and isobaric tagging for quantitation.

Conclusions:

  • GeLC-MS/MS is an invaluable technique for comprehensive proteomic analyses.
  • It provides robust identification and quantitation of proteins and post-translational modifications.
  • The method is adaptable for diverse biological samples.