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Electroporation by subnanosecond pulses.

Iurii Semenov1, Shu Xiao2, Andrei G Pakhomov1

  • 1Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA.

Biochemistry and Biophysics Reports
|August 3, 2016
PubMed
Summary
This summary is machine-generated.

Picosecond electric pulses (psEP) were explored for their effects on cell membranes. psEP treatment increased cell membrane conductance and depolarization, showing similar effects to longer pulses despite different mechanisms.

Keywords:
ElectropermeabilizationElectroporationMembrane permeabilityNanoporesPatch clampPicosecond pulses

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Area of Science:

  • Cellular electrophysiology
  • Membrane biophysics
  • Nanosecond and picosecond pulsed electric fields

Background:

  • Extensive research exists on micro- and nanosecond electric pulse effects on cell membranes.
  • The impact of picosecond electric pulses (psEP) on cell membrane electropermeabilization remains largely uninvestigated.

Purpose of the Study:

  • To characterize the plasma membrane effects of 500-picosecond electric stimuli.
  • To compare psEP effects with those of longer duration pulses.

Main Methods:

  • Whole-cell patch clamp electrophysiology.
  • Di-8-ANEPPS voltage-sensitive dye imaging.
  • Application of single and burst 500-picosecond electric pulses to rat hippocampal neurons (RHN), NG108, and CHO cells.

Main Results:

  • A single 500-ps pulse at 190 kV/cm increased membrane conductance and depolarized cells.
  • Effects were enhanced by pulse bursts but not significantly affected by pulse repetition rate (8 Hz - 1 kHz).
  • Cells exhibited inward rectification and slow conductance recovery (approx. 2 min), similar to nanosecond pulse effects.

Conclusions:

  • Picosecond electric pulses induce cell membrane permeabilization.
  • The observed permeabilization pattern suggests similar structural membrane rearrangements occur despite differing charging mechanisms between psEP and nsEP.