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IN VITRO MEASUREMENT OF TOTAL ANTIOXIDANT CAPACITY OF CRATAEGUS MACRACANTHA LODD LEAVES
Summary
The leaves of Crataegus macracantha Lodd possess significant antioxidant properties. This study demonstrates their ability to capture free radicals in vitro, suggesting potential pharmacological applications.
Area of Science:
- Pharmacognosy
- Phytochemistry
- Medicinal Plant Research
Background:
- Crataegus macracantha Lodd is a rare European plant species.
- Its pharmacological activities are less explored compared to Crataegus monogyna.
Purpose of the Study:
- To evaluate the in vitro free radical scavenging capacity of an extract from Crataegus macracantha Lodd leaves.
- To establish an analytical method for assessing its antioxidant potential.
Main Methods:
- Spectrophotometric analysis of chlorpromazine radical cation absorbance.
- Lyophilized leaf extract of Crataegus macracantha Lodd was tested.
- Ascorbic acid was used as a reference antioxidant.
Main Results:
- The study quantified the decrease in radical cation absorbance upon addition of the extract.
- Antioxidant activity was correlated with ascorbic acid concentration via calibration curves.
- Percentage inhibition of absorbance was calculated at specific time intervals.
Conclusions:
- The leaves of Crataegus macracantha Lodd exhibit notable antioxidant activity.
- Optimized analytical methods allow for rapid evaluation of antiradical potential with minimal reagent use.
