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Plasma functionalization procedure for antibody immobilization for SU-8 based sensor.

Immacolata Angelica Grimaldi1, Genni Testa1, Gianluca Persichetti1

  • 1Institute for Electromagnetic Monitoring of the Environment (IREA), National Research Council (CNR), Naples, Italy.

Biosensors & Bioelectronics
|August 4, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces an improved oxygen plasma treatment for immobilizing antibodies onto SU-8 surfaces, enhancing protein adsorption by 20% for better immunosensor performance.

Keywords:
BiosensingImmunoglobulin GMicroring resonatorOptical biosensorSurface immobilization

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Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Chemistry

Background:

  • Surface functionalization is crucial for biosensor development.
  • Optimizing antigen/antibody immobilization on polymeric substrates like SU-8 is essential for sensitive detection.

Purpose of the Study:

  • To develop and validate a novel oxygen plasma treatment protocol for efficient antigen/antibody immobilization on SU-8 layers.
  • To investigate the impact of plasma treatment parameters on SU-8 surface properties and protein binding.

Main Methods:

  • Oxygen plasma treatment of SU-8 layers at varying power and duration.
  • Surface characterization using Raman spectroscopy and Atomic Force Microscopy (AFM).
  • Immobilization efficiency assessed via fluorescence microscopy and label-free optical ring resonators.

Main Results:

  • Oxygen plasma treatment increased SU-8 surface roughness and altered functional groups.
  • Enhanced IgG immobilization by approximately 20% with good surface distribution repeatability.
  • Achieved label-free detection limits of 0.86 ng/cm² using SU-8 optical ring resonators.

Conclusions:

  • The proposed oxygen plasma protocol significantly improves IgG immobilization on SU-8.
  • This method offers a versatile approach for developing high-performance immunosensors using various antigen/antibody assays and polymeric materials.