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Freeze-fracture of 3T3 and HeLa cells for high-resolution SEM and deep-etch replicas: structure of the interphase

G H Haggis1

  • 1Canada Department of Agriculture, Ottawa.

Progress in Clinical and Biological Research
|January 1, 1989
PubMed
Abstract

No abstract available in PubMed .

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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