Related Experiment Video

Updated: Mar 16, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
07:00

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Published on: April 1, 2019

10.5K

Thromboxane A2 receptor (TBXA2R) is a potent survival factor for triple negative breast cancers (TNBCs)

Katy Orr1, Niamh E Buckley1, Paula Haddock1

  • 1Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast, UK.

Oncotarget
|August 4, 2016
PubMed

Insights

Thromboxane A2 receptor (TBXA2R) is elevated in good-outcome Triple Negative Breast Cancer (TNBC). Targeting TBXA2R shows promise for treating TNBC by inhibiting cell survival and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple Negative Breast Cancer (TNBC) presents the poorest clinical outcomes among breast cancer subtypes.
  • TNBC is characterized by the absence of Estrogen Receptor alpha (ERα), Progesterone Receptor (PR), and Human Epidermal growth factor Receptor 2 (HER2) expression.
  • Identifying novel therapeutic targets is crucial for improving TNBC patient prognosis.

Purpose of the Study:

  • To identify genes that drive proliferation and/or survival in TNBC.
  • To discover potential novel therapeutic targets for TNBC treatment.

Main Methods:

  • Microarray profiling of primary TNBC samples.
  • Differential gene expression analysis based on clinical outcomes (relapse within 3 years vs. no relapse > 3 years) after FEC chemotherapy.
  • Investigated the role of Thromboxane A2 receptor (TBXA2R) in TNBC cell lines, including knockdown experiments and analysis of its regulation by BRCA1 and c-Myc.

Main Results:

  • Elevated TBXA2R expression was observed in TNBCs with a good clinical outcome.
  • TBXA2R knockdown led to significant cell killing in TNBC cell lines.
  • TBXA2R expression is upregulated by BRCA1 knockdown, with c-Myc mediating this transcriptional repression.
  • TBXA2R promotes TNBC cell migration, invasion, and activates Rho signaling, which can be inhibited by ROCK inhibitors.
  • TBXA2R confers resistance to DNA damage by reducing reactive oxygen species levels.

Conclusions:

  • TBXA2R is a novel gene associated with breast cancer, essential for the survival and migration of a subset of TNBC.
  • TBXA2R represents a potential therapeutic target for developing more effective treatments for Triple Negative Breast Cancer.
Keywords:
BRCA1TBXA2RTNBCcell survivalpredictive marker

More Related Videos

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.3K
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

19.3K

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.0K
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
63
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.9K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.8K