Related Experiment Video
Updated: Mar 16, 2026
Ultrasonography in Experimental Reproductive Investigations on Rats
Published on: December 2, 2017
Measurement of testicular volume in Wistar rats using a caliper and ultrasonography in experimental surgery
Salomão Brêtas1, Edson Samesima Tatsuo2, Monica Odete Oliveira Brêtas3
1Fellow PhD degree, Postgraduate Program in Sciences Applied to Surgery and Ophthalmology, School of Medicine, Universidade Federal de Minas Gerais (UFMG), Brazil. Concept, design, and intellectual content of the study; manuscript preparation; technical procedures.
Purpose:
To compare the measurement of the testicular volume of Wistar rats using a caliper and ultrasonography.
Methods:
Forty Wistar rats were randomly assigned into four groups. A tensile force of 1.6 Newton (N) and 1.0 N was applied to the right spermatic cord in group I and group II, respectively. Group III was the sham group, and group IV served as a control. The initial and final testicular volumes were measured using a caliper and ultrasonography and compared. A significance level of 5% was used.
Results:
The Kappa coefficient was equal to 0.292 (p = 0.006). The Pearson correlation coefficient obtained for the percent reduction in the right testicular volume using ultrasonography and a caliper was equal to 0.696 (p < 0.001).
Conclusion:
There was reasonable agreement and a significant positive correlation between the percent reduction in the right testicular volume using ultrasonography and a caliper.
