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Related Concept Videos

Gap Junctions01:37

Gap Junctions

58.1K
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
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Gap Junctions01:27

Gap Junctions

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The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...
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Contact-dependent Signaling01:19

Contact-dependent Signaling

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Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
48.3K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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Antiepileptic Drugs: GABAergic Pathway Potentiators01:18

Antiepileptic Drugs: GABAergic Pathway Potentiators

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γ-aminobutyric acid or GABA, plays a pivotal role as an inhibitory neurotransmitter in the brain. GABA pathway potentiators, also known as GABAergic drugs, are a class of pharmaceutical agents designed to enhance the functioning of the GABAergic system. These medications primarily treat epilepsy, a neurological disorder characterized by recurrent seizures.
The key GABA pathway potentiators used in epilepsy management are as follows.
Benzodiazepines are a well-known class of drugs used for...
1.6K
Antiarrhythmic Drugs: Class III Agents as Potassium Channel Blockers01:12

Antiarrhythmic Drugs: Class III Agents as Potassium Channel Blockers

2.8K
Class III antiarrhythmic drugs are a group of medications that can prolong action potentials in the heart. They achieve this by blocking potassium channels or enhancing inward currents from sodium channels. However, these drugs have a unique property of "reverse use-dependence," which is most pronounced at slower heart rates and can lead to torsades de pointes—a specific type of arrhythmia. However, it is essential to note that excessive QT interval prolongation—a measure of...
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Related Experiment Video

Updated: Mar 16, 2026

An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
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An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay

Published on: February 1, 2019

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Terbinafine inhibits gap junctional intercellular communication.

Ju Yeun Lee1, Sei Mee Yoon2, Eun Ju Choi1

  • 1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon 21983, Republic of Korea.

Toxicology and Applied Pharmacology
|August 4, 2016
PubMed
Summary

Terbinafine, an antifungal drug, was found to inhibit gap junctional intercellular communication (GJIC) in cells. This antifungal agent

Keywords:
Gap junctional intercellular communicationI-YFP GJIC assayNaftifineTerbinafine

More Related Videos

Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

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Related Experiment Videos

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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

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Area of Science:

  • Pharmacology
  • Cell Biology
  • Biochemistry

Background:

  • Terbinafine is a well-established antifungal medication.
  • It functions by inhibiting squalene epoxidase, a key enzyme in fungal sterol synthesis.
  • Gap junctional intercellular communication (GJIC) plays a crucial role in cellular coordination.

Purpose of the Study:

  • To investigate the impact of terbinafine on GJIC.
  • To elucidate the mechanism by which terbinafine affects GJIC.

Main Methods:

  • Utilized fluorescence recovery after photobleaching (FRAP) and I-YFP GJIC assays.
  • Examined FRT-Cx43 and LN215 cell lines.
  • Assessed Cx43 phosphorylation and intracellular calcium levels.

Main Results:

  • Terbinafine demonstrated a reversible, dose-dependent inhibition of GJIC.
  • No significant changes in Cx43 phosphorylation or intracellular Ca(2+) were observed.
  • Naftifine, a related compound, also inhibited GJIC, but epigallocatechin gallate did not.

Conclusions:

  • Terbinafine inhibits GJIC through a novel mechanism.
  • The observed effect is specific to terbinafine and not a general property of squalene epoxidase inhibitors.
  • Further research is needed to fully understand the molecular basis of terbinafine-induced GJIC inhibition.