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Evolutionary and functional analysis of mulberry type III polyketide synthases
Han Li1, Jiubo Liang1, Hu Chen1
1State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, People's Republic of China.
BMC Genomics
|August 5, 2016
Summary
Researchers identified 18 type III polyketide synthase genes in mulberry plants, including chalcone synthases (CHS) and stilbene synthases (STS). This study provides targets for improving mulberry secondary metabolites and stress tolerance.
Area of Science:
- Plant biochemistry and molecular biology
- Genomics and gene evolution
Background:
- Type III polyketide synthases (PKS) are crucial for synthesizing plant polyphenols like flavonoids.
- Mulberry plants are rich in polyphenols, but their type III PKS genes remain largely uncharacterized.
- Understanding these genes can aid in genetic improvement for enhanced secondary metabolites and stress resistance.
Purpose of the Study:
- To identify and characterize type III PKS genes in mulberry (Morus spp.).
- To investigate the evolutionary history and functional roles of mulberry CHS and STS genes.
- To provide a foundation for future research on mulberry secondary metabolite biosynthesis.
Main Methods:
- Bioinformatic identification of 18 type III PKS genes (6 CHS, 10 STS, 2 PKS).
- Functional characterization via heterologous expression in E. coli.
- Microsynteny analysis to study gene duplication events.
- Evolutionary and expression analyses.
Main Results:
- Identified 18 type III PKS genes, including six MnCHS and ten MnSTS.
- Demonstrated that four characterized genes produce naringenin (from MnCHS) and resveratrol (from MnSTS).
- Segmental and tandem duplications expanded MnCHS and MnSTS families; evolutionary analysis revealed purifying selection and transcriptional subfunctionalization.
- Mulberry leaves rapidly convert resveratrol to oxyresveratrol upon UV-C irradiation.
Conclusions:
- Characterization of mulberry type III PKS genes is essential for understanding their function and evolution.
- This study lays the groundwork for manipulating these genes to enhance mulberry's valuable secondary metabolites.
- The findings contribute to the broader knowledge of plant polyphenol biosynthesis and stress response mechanisms.
Keywords:
Chalcone synthaseEvolutionary analysisFunctional analysisGene expressionMulberryStilbene synthaseMore Related Videos
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