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Updated: Mar 16, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Characterization of Vibrio cholerae Strains Isolated from the Nigerian Cholera Outbreak in 2010
Susann Dupke1, Kehinde A Akinsinde2, Roland Grunow1
1Robert Koch Institute, Highly Pathogenic Microorganisms (ZBS 2), Berlin, Germany.
Abstract:
We examined clinical samples from Nigerian patients with acute watery diarrhea for Vibrio cholerae during the 2010 cholera outbreak. A total of 109 suspected isolates were characterized, but only 57 V. cholerae strains could be confirmed using multiplex real-time PCR as well as rpoB sequencing and typed as V. cholerae O:1 Ogawa biotype El Tor. This finding highlighted the need for accurate diagnosis of cholera in epidemic countries to implement life-saving interventions.
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