Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.7K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.7K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

2.4K
2.4K
Inflammatory Response01:28

Inflammatory Response

18.1K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
18.1K
T Cell Types and Functions01:24

T Cell Types and Functions

3.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

13.7K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.7K
Inflammation01:38

Inflammation

63.8K
Overview
63.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The importance of meal timing for maintenance of daily rhythms in the gut transcriptome and microbiota.

Npj biological timing and sleep·2026
Same author

Human MASLD is a diurnal disease driven by multisystem insulin resistance and reduced insulin availability at night.

Cell metabolism·2026
Same author

Clocks on steroids: how glucocorticoid receptors tell cells the time.

The Journal of endocrinology·2026
Same author

Working against the clock: does night shift work drive multisystem disease?

European journal of preventive cardiology·2026
Same author

Glucocorticoid-dependence and independence of the circadian liver transcriptome.

Npj biological timing and sleep·2026
Same author

Associations between shift work patterns and sleep disturbance: an analysis of cross-sectional data from UK Biobank.

BMJ open·2026

Related Experiment Video

Updated: Mar 16, 2026

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
06:50

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Published on: July 24, 2018

8.0K

The circadian clock regulates inflammatory arthritis.

Laura E Hand1, Thomas W Hopwood1, Suzanna H Dickson1

  • 1Faculty of Biology, Medicine and Health, University of Manchester and Manchester Academic Health Sciences Centre, Manchester, United Kingdom.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|August 5, 2016
PubMed
Summary

The biological clock actively suppresses inflammatory arthritis during the night. Disrupting the circadian clock worsens inflammation, but targeting core clock proteins like cryptochrome (CRY) shows therapeutic potential for inflammatory diseases.

Keywords:
cryptochromediurnalfibroblast-like synoviocyterheumatoid arthritis

More Related Videos

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

1.4K
Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
09:18

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Published on: February 24, 2023

10.7K

Related Experiment Videos

Last Updated: Mar 16, 2026

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
06:50

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Published on: July 24, 2018

8.0K
An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
07:37

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

1.4K
Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
09:18

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Published on: February 24, 2023

10.7K

Area of Science:

  • Chronobiology
  • Immunology
  • Rheumatology

Background:

  • Chronic inflammatory diseases exhibit diurnal symptom variation.
  • Circadian clock disruption exacerbates inflammatory conditions like rheumatoid arthritis.

Purpose of the Study:

  • Investigate the mechanistic links between the biological clock and inflammatory arthritis pathways.
  • Determine the role of the molecular clock in regulating inflammation within affected tissues.

Main Methods:

  • Induced inflammatory arthritis in mice using collagen administration.
  • Monitored daily variations in paw swelling and inflammatory cytokine expression.
  • Assessed the impact of constant light exposure on circadian rhythm and inflammation.
  • Investigated the role of core clock proteins (CRY1/2) in fibroblast-like synoviocytes (FLSs).

Main Results:

  • Arthritis induction led to daily variations in limb inflammation, with repression of inflammatory markers during the dark phase.
  • An active molecular clock was identified in inflamed limbs, with FLSs identified as a source of rhythmic inflammatory signals.
  • Constant light exposure disrupted peripheral clocks and abolished nighttime inflammation repression.
  • Core clock proteins CRY1 and CRY2 were found to repress inflammation in FLSs, and a CRY activator demonstrated anti-inflammatory effects in human cells.

Conclusions:

  • The circadian clock actively represses inflammatory pathways during the dark phase in chronic inflammatory conditions.
  • The interaction between the circadian clock and inflammation presents a potential therapeutic target for inflammatory diseases.
  • Core clock proteins, such as CRY, possess anti-inflammatory properties with potential clinical applications.