Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sorting.

STAR protocols·2026
Same author

Retraction: JQ1 suppresses tumor growth through downregulating LDHA in ovarian cancer.

Oncotarget·2026
Same author

Correction: Evaluating AI guidelines in leading family medicine journals: a cross-sectional study.

BMC primary care·2026
Same author

Assessing patient risk, benefit, and outcomes in drug development: a decade of sorafenib clinical trials.

European journal of clinical pharmacology·2026
Same author

The IDEA Framework: A Consensus-based Model for Integrating Continuous Glucose Monitoring Into Pharmacy Practice for Diabetes Care.

Canadian journal of diabetes·2026
Same author

Bridging the Gap: A Mixed-Methods Evaluation of a New Rural Maternity Care Center Amid Nationwide Closures.

International journal of environmental research and public health·2026

Related Experiment Video

Updated: Mar 16, 2026

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
10:58

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

Published on: November 17, 2011

21.7K

A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry.

Leo Li-Ying Chan1, Tim Smith2, Kendra A Kumph2

  • 1Department of Technology R&D, Nexcelom Bioscience LLC, 360 Merrimack St. Building 9, Lawrence, MA, 01843, USA. lchan@nexcelom.com.

Cytotechnology
|August 5, 2016
PubMed
Summary

A new high-throughput method using acridine orange/propidium iodide (AO/PI) staining with the Celigo image cytometer enables rapid cell concentration and viability assessment. This AO/PI method significantly reduces assay time for high-throughput screening compared to traditional automated cell counters.

Keywords:
Acridine orangeCeligoConcentrationHigh-throughputImage cytometryPropidium iodideScreening assayViability

More Related Videos

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
10:44

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Published on: December 28, 2015

13.6K
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.9K

Related Experiment Videos

Last Updated: Mar 16, 2026

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
10:58

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

Published on: November 17, 2011

21.7K
Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
10:44

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Published on: December 28, 2015

13.6K
Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques
09:48

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

7.9K

Area of Science:

  • Cell Biology
  • Biotechnology
  • Assay Development

Background:

  • Accurate cell concentration and viability are crucial for normalizing cell-based assay data.
  • Traditional automated cell counters are impractical for high-throughput screening due to single-sample limitations.
  • Plate-based image cytometry offers a potential solution for high-throughput biomolecular screening.

Purpose of the Study:

  • To develop and validate a high-throughput acridine orange/propidium iodide (AO/PI) based method for cell concentration and viability determination.
  • To assess the performance of this method using the Celigo image cytometer for various assay formats.

Main Methods:

  • Utilized the Celigo image cytometer for high-throughput AO/PI staining and cell counting.
  • Validated the method against a Cellometer automated cell counter.
  • Determined the dynamic range and consistency of cell concentration and viability measurements.

Main Results:

  • The high-throughput AO/PI method successfully determined cell concentration and viability.
  • Analysis of 96-well to 384-well plates was completed in under 7 minutes.
  • The method demonstrated good dynamic range and consistency.

Conclusions:

  • The developed high-throughput AO/PI method provides a faster and more efficient alternative for cell concentration and viability assessment in high-throughput screening.
  • This method enhances efficiency for assays requiring multiple cell count and viability measurements prior to downstream applications.