[Differentiation of pancreatic lipase and pseudolipase serum activity]

The determination of the serum lipase activity by turbidimetric assay was modified and false-positive results due to pseudolipase presence were eliminated. The new method includes a preincubation with beta-mercaptoenthanol and its conditions are specified to use with kinetic analysers as LKB 2086. By using our new method we estimated the activity of the pseudolipase in 36.4% of the group of 85 patients with elevated serum lipase activity determined by standard turbidimetry. By introducing our differential method with beta-mercaptoethanol correction the increasing correlation about 27% between lipase activity and serum alpha-amylase activity was estimated.