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Updated: Mar 16, 2026
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
Published on: October 30, 2015
Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry
Jonathan Shintaku1,2,3, Denis C Guttridge4,5
1Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University College of Medicine, 460 W. 12th Avenue, Columbus, OH, 43210, USA.
Abstract:
Mitochondrial function is a key component of skeletal muscle health, and its dysfunction has been associated with a wide variety of diseases. Microplate-based respirometry measures aerobic respiration of live cells through extracellular changes in oxygen concentration. Here, we describe a methodology to measure aerobic respiration of intact murine skeletal muscle tissue. The tissues are not cultured, permeabilized, or enzymatically dissociated to single fibers, so there is minimal experimental manipulation affecting the samples prior to acquiring measurements.
