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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Regulated mRNA Transport

Regulated mRNA Transport

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Related Experiment Video

Updated: Mar 16, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Sec16 alternative splicing dynamically controls COPII transport efficiency.

Ilka Wilhelmi¹, Regina Kanski¹, Alexander Neumann¹

¹Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Institute of Chemistry and Biochemistry, Laboratory of RNA Biochemistry, Takustrasse 6, 14195 Berlin, Germany.

Nature Communications

|August 6, 2016

Summary

This summary is machine-generated.

Alternative splicing of Sec16 regulates COPII vesicle transport, adapting protein secretion to cellular needs. This discovery reveals a new layer of control for secretory pathways.

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Last Updated: Mar 16, 2026

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Area of Science:

Cell Biology
Molecular Biology
Protein Secretion

Background:

Secretory protein transport from the endoplasmic reticulum (ER) to the Golgi relies on COPII-coated vesicles.
Regulation of COPII transport for varying cargo loads and identities remains poorly understood.

Purpose of the Study:

To investigate how alternative splicing of Sec16 regulates COPII transport in response to T-cell activation.
To elucidate the molecular mechanisms by which Sec16 isoforms influence COPII dynamics and cargo transport.

Main Methods:

Utilized splice-site blocking morpholinos and CRISPR/Cas9 genome engineering.
Analyzed the impact of Sec16 alternative splicing on ER exit sites, COPII vesicle dynamics, and transport efficiency.

Main Results:

Demonstrated that Sec16 alternative splicing controls the number of ER exit sites and COPII dynamics.
Showed that different Sec16 isoforms exhibit distinct abilities to recruit COPII components.
Established a link between Sec16 splicing and adaptation of COPII transport to increased secretory cargo.

Conclusions:

Sec16 alternative splicing acts as a crucial regulatory mechanism for COPII-mediated protein transport.
The C-terminal domain of Sec16 functions as a splicing-controlled platform for protein interactions.
This study introduces alternative splicing as a novel regulatory layer for protein secretion and cellular adaptation.