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Updated: Mar 16, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
H-NS: an overarching regulator of the Vibrio cholerae life cycle
Julio C Ayala1, Anisia J Silva2, Jorge A Benitez2
1Morehouse School of Medicine, Department of Microbiology, Biochemistry and Immunology, Atlanta, GA, USA; University of Alabama at Birmingham, Department of Microbiology, Birmingham, AL, USA.
Abstract:
Vibrio cholerae has become a model organism for studies connecting virulence, pathogen evolution and infectious disease ecology. The coordinate expression of motility, virulence and biofilm enhances its pathogenicity, environmental fitness and fecal-oral transmission. The histone-like nucleoid structuring protein negatively regulates gene expression at multiple phases of the V. cholerae life cycle. Here we discuss: (i) the regulatory and structural implications of H-NS chromatin-binding in the two-chromosome cholera bacterium; (ii) the factors that counteract H-NS repression; and (iii) a model for the regulation of the V. cholerae life cycle that integrates H-NS repression, cyclic diguanylic acid signaling and the general stress response.
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