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Updated: Mar 16, 2026
Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Use of panel testing for detection of antinuclear antibody in a resource-limited setting: an appraisal
Ranjana Walker Minz1, Yashwant Kumar1, Biman Saikia1
1a Department of Immunopathology , Post Graduate Institute of Medical Education & Research , Chandigarh , India.
Objectives:
Despite an increase in the incidence of systemic connective tissue diseases (CTD), panel testing for detection of antinuclear antibodies (ANA) is not a routine practice in many health centers of the Indian subcontinent. Consequently, the data on its significance is scanty.
Methods:
To evaluate utility of panel testing, line immunoassay (LIA) and indirect immunofluorescence antinuclear antibody test (IIF-ANA) were performed in 321 cases of CTD.
Results:
Out of 321 serum samples screened by the above tests, 227 were positive and 18 were negative by both LIA and IIF-ANA. Additional 11/321 (3.4%) cases were picked up by LIA. SSA was most common specificity in these cases followed by SSA/SSB, SSB, Ro-52, Jo-1, dsDNA and nRNP/Sm.
Conclusion:
Use of LIA along with IF-ANA and ELISA improves sensitivity of CTD screening.
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