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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Development and application of a general plasmid reference material for GMO screening.

Yuhua Wu¹, Jun Li¹, Yulei Wang¹

¹Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China; Supervision and Test Center (Wuhan) for Environmental Safety of Genetically Modified Plants, Ministry of Agriculture, No. 2 Xudong 2nd Road, Wuhan 430062, China.

|August 8, 2016

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A new general reference plasmid, pBI121-Screening, was developed for genetically modified organism (GMO) detection. This tool enhances screening tests by providing a reliable positive control, reducing workload and costs.

Area of Science:

Biotechnology
Molecular Biology
Genetics

Background:

Analytical controls and taxon-specific sequences are crucial for reliable GMO detection.
Current methods often lack a universal positive control, increasing complexity and cost.

Purpose of the Study:

To construct a general reference plasmid (pBI121-Screening) for GMO detection screening.
To validate the suitability of this plasmid as a positive control in PCR-based assays.

Main Methods:

Co-integration of 11 common genetic elements (promoters, marker genes, terminators) and crop reference gene fragments into a single plasmid.
Suitability testing using PCR amplification efficiency and sensitivity assays.

Main Results:

Keywords:

GMO General positive control Genetic element Reference gene Screening

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Last Updated: Mar 16, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

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High-throughput Yeast Plasmid Overexpression Screen

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The pBI121-Screening plasmid did not interfere with PCR amplification efficiencies for screening and taxon-specific methods.

Assays demonstrated high sensitivity, detecting as few as 5-10 copies of the plasmid.

The plasmid effectively serves as a general positive control.

Conclusions:

The pBI121-Screening plasmid provides a standardized and efficient positive control for GMO detection.
Its development addresses the need for a universal control, simplifying GMO screening and reducing costs.