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Updated: Mar 16, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Pontibacter rugosus sp. nov., isolated from seawater
Dae In Kim1, Ji Hee Lee1, Joo Won Kang1
1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea.
Abstract:
A motile and rod-shaped bacterium, designated strain KYW1030T, was isolated from seawater collected from the Gwangyang bay (Republic of Korea). Cells were Gram-reaction-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0 and iso-C17 : 0 3-OH. The strain contained MK-7 as the major isoprenoid quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids and sym-homospermidine as the major polyamine. The DNA G+C content was 46 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW1030T forms an evolutionary lineage within the radiation enclosing the members of the genus Pontibacter, with Pontibacterakesuensis AKS 1T (97.9 % 16S rRNA gene sequence similarity) as its nearest neighbour. A number of phenotypic characteristics distinguished strain KYW1030T from the related members of the genus Pontibacter. On the basis of the evidence presented in this study, a novel species, Pontibacter rugosus sp. nov., is proposed for strain KYW1030T (=KACC 18739T=JCM 31319T).
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