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Related Experiment Video

Updated: Mar 16, 2026

Isolating Mesangiogenic Progenitor Cells MPCs from Human Bone Marrow
09:53

Isolating Mesangiogenic Progenitor Cells MPCs from Human Bone Marrow

Published on: July 15, 2016

8.7K

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow.

Marina Montali1, Serena Barachini1, Simone Pacini2

  • 1Department of Clinical and Experimental Medicine, University of Pisa.

Journal of Visualized Experiments : Jove
|August 9, 2016
PubMed
Summary

Researchers discovered a new cell type in human bone marrow, termed Mesangiogenic Progenitor Cells (MPCs), which have distinct properties from Mesenchymal Stromal Cells (MSCs) and possess both mesenchymal and angiogenic potential.

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Cellular and molecular medicine

Background:

  • Human bone marrow (hBM)-derived Mesenchymal Stromal Cells (MSCs) are crucial for clinical applications.
  • Existing protocols aim to isolate MSCs, but novel cell populations may be overlooked.

Purpose of the Study:

  • To isolate and characterize a novel cell population from hBM distinct from MSCs.
  • To establish a reproducible protocol for their isolation and differentiation.
  • To propose a new nomenclature for these cells.

Main Methods:

  • Isolation of cells cultured in human serum-supplemented medium.
  • Characterization using morphology, adherence properties, Ki-67 staining, and flow cytometry (CD73, CD90, CD31, CD45).
  • Assessment of nestin expression, F-actin organization, and differentiation potential (mesengenic and angiogenic).

Main Results:

  • A novel cell population, initially named Mesodermal Progenitor Cells (MPCs), was identified with unique characteristics.
  • MPCs exhibit slow cycling, strong adherence, and distinct surface markers compared to MSCs.
  • MPCs demonstrate both mesenchymal differentiation capacity and angiogenic potential, supporting their multipotent nature.
  • An optimized protocol for isolation and characterization was developed.
  • The proposed new name is Mesangiogenic Progenitor Cells.

Conclusions:

  • A distinct progenitor cell population, Mesangiogenic Progenitor Cells (MPCs), has been identified in human bone marrow.
  • MPCs possess multipotent differentiation capabilities, including mesenchymal and angiogenic potential.
  • An optimized protocol facilitates their isolation, characterization, and differentiation for potential therapeutic use.