Related Experiment Video
Updated: Jul 28, 2026

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
[The dependence of the carcinogenic effect on the concentration of N-nitrosodiethylamine]
Abstract:
In a 86-week-long experiment using 1087 mice (487 hybrids and 600 non-inbred animals), the concentration-effect relationship was studied for N-nitrosodiethylamine (NDEA) given in drinking water. NDEA concentrations of 0.08; 0.4; 2.0 and 10.0 mg/l produced tumors in male and female hybrids with the frequencies of 35.9/30.9, 49.9/43.6, 61.9/72.3 and 81.8/82.9%, respectively (20.0.20.0% in controls) whereas in non-inbred animals the frequencies were 15.6/34.2, 24.2/33.3, 35.9/57.1 and 78.4/80.9%, respectively (15.0/30.0% in controls). The "minimal effective" carcinogenic level of NDEA proved 0.4 mg/l for hybrids and 2.0 mg/l for non-inbred mice (P less than 0.05). No sex differences were found for frequency or histologic pattern of tumor. The data obtained can be used in calculating sanitary-hygienic standards for NDEA.
More Related Videos
09:33Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
Related Concept Videos
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
2° Amines to N-Nitrosamines: Reaction with NaNO2
Physical Properties of Amines
Aldehydes and Ketones with Amines: Enamine Formation Mechanism
Mutagenicity and Carcinogenicity