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Updated: Mar 16, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering.

Hui Min Tay¹, David C Yeo², Christian Wiraja²

¹Lee Kong Chian School of Medicine, Nanyang Technological University.

Journal of Visualized Experiments : Jove

|August 9, 2016

Summary

This summary is machine-generated.

Dean Flow Fractionation (DFF) efficiently separates nanoparticle-labeled cells from unbound particles using microfluidics. This method enhances cell purification for improved bioimaging and clinical applications.

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Last Updated: Mar 16, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Area of Science:

Biotechnology
Cell Engineering
Microfluidics

Background:

Cell engineering with micro/nanoparticles (NPs) is vital for therapeutics, bioimaging, and phenotype control.
Unbound NPs after cell labeling cause bioimaging noise and affect non-target cells.
Conventional centrifugation is insufficient for removing residual unbound NPs.

Purpose of the Study:

To develop an efficient, high-throughput method for separating labeled cells from free NPs.
To address the challenge of unbound nanoparticles in cell engineering protocols.
To enable interference-free clinical applications of engineered cells.

Main Methods:

Utilized Dean Flow Fractionation (DFF), an inertial microfluidics-based strategy.
Employed a spiral microdevice for continuous cell collection and buffer exchange.
Applied size-based separation to distinguish between labeled cells and free NPs.

Main Results:

>90% cell recovery (THP-1, MSCs) achieved with continuous collection.
>95% depletion of unbound fluorescent dye or NPs (silica, PLGA).
High cell processing throughput of 10^6 cells/min demonstrated.

Conclusions:

DFF offers a single-step, size-based purification for nanoparticle-engineered cells.
This method significantly reduces background noise and unintended cellular effects.
The DFF strategy is highly valuable for large-volume cell purification in clinical settings.