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Long noncoding RNA XIST acts as an oncogene in non-small cell lung cancer by epigenetically repressing KLF2

Jing Fang1, Cheng-Cao Sun2, Cheng Gong3

  • 1Department of Oncology, Wuhan Pu-Ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430034, PR China.

Biochemical and Biophysical Research Communications
|August 10, 2016
PubMed

Insights

Long noncoding RNAs (lncRNAs) like XIST promote non-small cell lung cancer (NSCLC) growth. Silencing XIST inhibits NSCLC progression by regulating KLF2 expression, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are key regulators in cancer.
  • XIST, an oncogenic lncRNA in other cancers, has an unknown role in non-small cell lung cancer (NSCLC).

Purpose of the Study:

  • To investigate the role and mechanism of XIST in NSCLC tumorigenesis.

Main Methods:

  • Quantitative real-time PCR to assess XIST expression.
  • In vitro assays (proliferation, migration, invasion) and in vivo tumorigenicity assays.
  • RNA immunoprecipitation (RIP) and RNA pull-down assays to identify molecular interactions.
  • Rescue experiments to validate mechanistic findings.

Main Results:

  • XIST was over-expressed in NSCLC tissues and correlated with poor prognosis.
  • XIST knockdown suppressed NSCLC cell proliferation, migration, invasion, and in vivo tumor growth.
  • XIST directly interacted with EZH2 to suppress KLF2 transcription.
  • KLF2 silencing partially mediated XIST's oncogenic functions.

Conclusions:

  • XIST acts as an oncogenic lncRNA in NSCLC by suppressing KLF2 via EZH2.
  • XIST is a potential therapeutic target for NSCLC treatment.
Keywords:
KLF2Long noncoding RNA XISTNon-small cell lung cancer (NSCLC)ProliferationTumorigenesis

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