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Updated: Mar 16, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Identification of nontuberculous mycobacteria in MGIT by matrix-assisted laser desorption/ionization mass
Jung-Yien Chien1,2,3, Chong-Jen Yu1,2,3, Po-Ren Hsueh1,2,3
1Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
Aim:
The performance of Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in identifying species of nontuberculous mycobacteria (NTM) in flagged Mycobacteria Growth Indicator Tubes (MGIT)s is unclear.
Materials & Methods:
A total of 92 sequential MGIT-positive nontuberculous mycobacteria isolates, including Mycobacterium intracellulare, M. abscessus complex, M. avium complex and M. avium, were used to compare the performance of the MALDI-TOF MS for species-level identification with that of the BluePoint MycoID plus kit and final identification.
Results:
A total of 44 (47.8%) and 80 (87.0%) isolates were correctly identified to the species level by the MALDI-TOF MS and BluePoint MycoID plus kit, respectively.
Conclusion:
The procedure of MALDI-TOF MS in MGITs inoculated with clinical specimens is needed to be further optimized.
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