Thymic E4bp4 gene transcription is up-regulated in the chicken during experimental peritonitis modified by the
Elzbieta Turkowska1, Dominika Pietruszka1, Krystyna Skwarlo-Sonta1
1Department of Animal Physiology, Institute of Zoology, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warsaw, Poland.
Abstract:
Immunity, like other vertebrate processes, undergoes the diurnal and seasonal rhythmicity generated and synchronized by the endogenous clock. In the present study a transcription of the selected clock genes was evaluated in the chicken thymus to verify its supposed role as a peripheral clock and to check its relation with the seasonality of immune function. Chickens kept from hatch in the season-related lighting conditions (LD 16:8 in summer vs 8:16 in winter) and in a controlled temperature were exposed to the experimental peritonitis elicited by i.p. thioglycollate injection. Previously described seasonality of the inflammatory response has been confirmed and the diurnal rhythms of a core clock gene Per3 and its repressor E4bp4 in the thymus has been evidenced. E4bp4 transcription was up-regulated in inflamed chickens while that of Per3 appeared independent of the locally induced inflammation. Our results suggest an interconnecting role of E4BP4 between molecular clock and immunity in the chicken.
Related Concept Videos
02:27Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
01:58Cell Specific Gene Expression