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BiDiFuse: a FIJI plugin for fusing bi-directionally recorded microscopic image volumes
Jan R Detrez1, Jean-Marie Vanderwinden2, Michaël Barbier1,3
1Laboratory of Cell Biology & Histology, Department of Veterinary Sciences, University of Antwerp, 2020 Antwerp, Belgium.
Abstract:
Deep tissue imaging is increasingly used for non-destructive interrogation of intact organs and small model organisms. An intuitive approach to increase the imaging depth by almost a factor of 2 is to record a sample from two sides and fuse both image stacks. However, imperfect three-dimensional alignment of both stacks presents a computational challenge. We have developed a FIJI plugin, called BiDiFuse, which merges bi-directionally recorded image stacks via 3D rigid transformations. The method is broadly applicable, considering it is compatible with all optical sectioning microscopes and it does not rely on fiducial markers for image registration.
Availability And Implementation:
The method is freely available as a plugin for FIJI from https://github.com/JanDetrez/BiDiFuse/ CONTACT: winnok.devos@uantwerpen.be.
