Related Experiment Video
Updated: Mar 16, 2026

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
Published on: December 14, 2019
Scoping the effectiveness and evolutionary obstacles in using plasmid-dependent phages to fight antibiotic resistance
Ville Ojala1,2, Sari Mattila1,2, Ville Hoikkala1,2
1Department of Biological & Environmental Science, Centre of Excellence in Biological Interactions, University of Jyväskylä, Jyväskylä, Finland.
Aim:
To investigate the potential evolutionary obstacles in the sustainable therapeutic use of plasmid-dependent phages to control the clinically important conjugative plasmid-mediated dissemination of antibiotic resistance genes to pathogenic bacteria.
Materials & Methods:
The lytic plasmid-dependent phage PRD1 and the multiresistance conferring plasmid RP4 in an Escherichia coli host were utilized to assess the genetic and phenotypic changes induced by combined phage and antibiotic selection.
Results & Conclusions:
Resistance to PRD1 was always coupled with either completely lost or greatly reduced conjugation ability. Reversion to full conjugation efficiency was found to be rare, and it also restored the susceptibility to plasmid-dependent phages. Consequently, plasmid-dependent phages constitute an interesting candidate for development of sustainable anticonjugation/antiresistance therapeutic applications.
Related Concept Videos
Lytic Cycle of Bacteriophages
Plasmids
Antibiotic Selection
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Development of Antibiotic Resistance
DNA Bacteriophages

