Joint toxicity prediction of nanoparticles and ionic counterparts: Simulating toxicity under a fate scenario

The joint effects of NPs with other chemicals is not fully yet understood along with the joint effects of the particulate and dissolved forms/fractions. The predictability of joint effects is of great importance for environmental risk assessment. Therefore this study aimed at inferring on the predictability of NPs binary mixture toxicity based on their ionic counterparts' mixtures, and evaluating if the joint toxicity of two forms of the same element (NP and ion) acts as dilution of each other. Effects of individual and mixtures of ZnO and Ag NPs and their respective salts (AgNO₃ and ZnCl₂) were studied in immobilization and feeding tests using Daphnia magna. NPs mixture toxicity patterns did not mirror their ionic counterparts' mixture toxicity responses and therefore their prediction should not rely on the available knowledge for regular chemicals. Regarding mixtures from the same element with different forms (NP and ions), both Zn and Ag mixtures showed a deviation from additivity, relying on the interaction between NP and ions. A synergistic effect was depicted when the concentrations of Ag ions increased, while antagonism was observed with AgNP increase in suspension. This is an expected pattern in long term studies due to dissolution, relating fate and toxicity.