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Updated: Mar 16, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Experimental demonstration of an adaptive architecture for direct spectral imaging classification
Abstract:
Spectral imaging is a powerful tool for providing in situ material classification across a spatial scene. Typically, spectral imaging analyses are interested in classification, though often the classification is performed only after reconstruction of the spectral datacube. We present a computational spectral imaging system, the Adaptive Feature-Specific Spectral Imaging Classifier (AFSSI-C), which yields direct classification across the spatial scene without reconstruction of the source datacube. With a dual disperser architecture and a programmable spatial light modulator, the AFSSI-C measures specific projections of the spectral datacube which are generated by an adaptive Bayesian classification and feature design framework. We experimentally demonstrate multiple order-of-magnitude improvement of classification accuracy in low signal-to-noise (SNR) environments when compared to legacy spectral imaging systems.
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