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Related Experiment Video

Updated: Mar 16, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Clostridium difficile Adhesins.

Séverine Péchiné¹, Cécile Denève-Larrazet¹, Anne Collignon²

¹Unité Bactéries Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, 92290, Châtenay Malabry, France.

Methods in Molecular Biology (Clifton, N.J.)

|August 11, 2016

Summary

Clostridium difficile adherence to host cells is crucial for intestinal infection. This study details methods to investigate bacterial colonization factors and their adhesive properties.

Keywords:

Adherence Adhesins Clostridium difficile Intestinal colonization Surface proteins

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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Area of Science:

Microbiology
Infectious Diseases
Gastroenterology

Background:

Clostridium difficile causes a range of intestinal diseases, from mild diarrhea to severe colitis.
Infection pathogenesis involves spore germination, bacterial multiplication, and toxin production (TcdA, TcdB, CDT).
Bacterial adhesins are key factors for C. difficile colonization in the gut.

Purpose of the Study:

To describe methods for studying Clostridium difficile adherence to host cells.
To present techniques for evaluating bacterial colonization in vivo.
To demonstrate the adhesive properties of specific surface proteins.

Main Methods:

In vitro assays using epithelial cells to assess C. difficile adherence.
In vivo studies in animal models to analyze intestinal colonization.
Methods to characterize adhesive surface proteins like Cwp66, GroEL, and FbpA.

Main Results:

Established protocols for in vitro and in vivo adherence studies.
Demonstrated adhesive capabilities of C. difficile surface proteins.
Provided a framework for identifying novel colonization factors.

Conclusions:

Adherence mechanisms are critical for C. difficile pathogenesis.
The described methods facilitate the study of bacterial colonization factors.
Understanding adherence is vital for developing therapeutic strategies against C. difficile infections.