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Updated: Mar 16, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Comment on "SUMO deconjugation is required for arsenic-triggered ubiquitylation of PML"
Omar Ferhi1, Laurent Pérès1, Sarah Tessier1
1Université Paris Diderot, Sorbonne Paris Cité, Institut Universitaire d'Hématologie, Hôpital Saint Louis, Paris 75010, France. INSERM UMR 944, Equipe Labellisée par la Ligue Nationale contre le Cancer, Hôpital Saint Louis, Paris 75010, France. CNRS UMR 7212, Hôpital Saint Louis, Paris 75010, France.
Abstract:
Fasci et al proposed that a SENP1-mediated switch from SUMO2 to SUMO1 conjugation on Lys(65) in promyelocytic leukemia protein (PML) is required for arsenic-induced PML degradation, the basis for the antileukemic activity of arsenic. We found that PML or PML/RARA (retinoic acid receptor α) mutants that cannot be SUMO-conjugated on this specific site nevertheless underwent immediate arsenic-triggered SUMO modification. Moreover, these mutants were efficiently degraded in cells and even in vivo, demonstrating that SUMOylation of Lys(65) was dispensable for arsenic response. The existence and putative role of a SUMO switch on PML should thus be reassessed.
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