Data in support for the measurement of serum 25-hydroxyvitamin D (25OHD) by tandem mass spectrometry
M E Jensen1, F M Ducharme2, Y Théorêt3
1Centre for Asthma and Respiratory Diseases, School of Biomedical Sciences & School of Medicine and Public Health, University of Newcastle, Callaghan, Australia.
Abstract:
This article provides data and a method related to a research paper entitled "Assessing vitamin D nutritional status: is capillary blood adequate?" (Jensen et al., 2016) [1]. Circulating 25OHD, the accepted biomarker of the vitamin D nutritional status, is routinely measured by automated immunoassays, that although may be performed in hospital central laboratories, often suffer from a lack of specificity with regards to the different vitamin D metabolites, "Measurement of circulating 25-hydroxyvitamin D: a historical review" (Le Goff et al., 2015) [2]. Mass spectrometry offers this specificity. This article describes the performance of an in-house tandem mass spectrometry method for the individual measurement of 25OHD3, 25OHD2 and 3-épi-25OHD3.
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