Data on publications, structural analyses, and queries used to build and utilize the AlloRep database

Area of Science:

• Molecular Biology
• Genetics
• Bioinformatics

Background:

• The LacI/GalR family comprises crucial transcription regulators.
• Understanding their function requires integrated data on sequence, structure, and mutations.
• Existing resources may lack comprehensive, easily accessible data.

Purpose of the Study:

• To create a centralized database, AlloRep, for the LacI/GalR transcription regulator family.
• To compile and present diverse data types including sequence, mutagenesis, and structural information.
• To facilitate research on LacI/GalR proteins through accessible data and query examples.

Main Methods:

• Compiled sequence alignments for over 3000 proteins across 45 subfamilies.
• Collected and curated phenotypic and biochemical data for nearly 6000 mutants from literature.
• Generated residue-contact networks for structural data of 65 proteins.

Main Results:

• The AlloRep database (www.AlloRep.org) now hosts extensive integrated data.
• Includes sequence alignments, detailed mutant data (oligomerization, stability, DNA binding, allostery), and structural networks.
• Provides example queries for database utilization.

Conclusions:

• AlloRep serves as a valuable, centralized resource for the LacI/GalR transcription regulator family.
• The integrated data promotes deeper understanding of protein function and regulation.
• Facilitates future research through accessible sequence, structural, and mutagenesis information.