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Updated: Mar 16, 2026
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
Comparative growth of spotted fever group Rickettsia spp. strains in Vero cells
Arannadia Barbosa Silva1,2, Myrian Morato Duarte3, Vinicius Figueiredo Vizzoni2,4
1Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Programa de Pós-Graduação em Biodiversidade e Saúde, Rio de Janeiro, RJ, Brasil.
Abstract:
In Brazil, the spotted fever group (SFG) Rickettsia rickettsii and Rickettsia parkeri related species are the etiological agents of spotted fever rickettsiosis. However, the SFG, Rickettsia rhipicephali, that infects humans, has never been reported. The study of growth dynamics can be useful for understanding the infective and invasive capacity of these pathogens. Here, the growth rates of the Brazilian isolates R. rickettsii str. Taiaçu, R. parkeri str. At#24, and R. rhipicephali HJ#5, were evaluated in Vero cells by quantitative polymerase chain reaction. R. rhipicephali showed different kinetic growth compared to R. rickettsii and R. parkeri.
