Structure of polysaccharide moiety of Pseudomonas xanthomarina KMM 1447T lipopolysaccharide
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Updated: Mar 16, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Maxim S Kokoulin1, Svetlana V Tomshich1, Anatoly I Kalinovsky1
1G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.
Abstract:
The structural analysis of a polysaccharide moiety of Pseudomonas xanthomarina KMM 1447T lipopolysaccharide (LPS) was carried out. Mild acid degradation of LPS resulted in identification of two polysaccharides. The major one was built of β-D-GlcpNAcA residues amidated with L-Ala and Gly residues randomly. The minor polysaccharide was composed of branched tetrasaccharide repeating units constituted by two D-Galр, D-GalpNAc and ether of D-Glc with (2R,4R)-2,4-dihydroxypentanoic acid lactone (2R,4R-Dhpl): →3)-α-D-Galр-(1 → 3)-β-D-Galр-(1 → 3)-[β-D-Glcp4(2R,4R-Dhpl)-(1 → 4)]-β-D-GalpNAc-(1 → .
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