Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair.

Nature communications·2026

Same author

Synthetic aptamer mechanoreceptors enable cell-specific force sensing and temporal control via DNA circuits.

Nature communications·2026

Same author

A Modular Toolkit for Nanoscale Interrogation of Multiprotein Assemblies Inside Living Cells.

ACS nano·2026

Same author

Hour-scale single-molecule imaging reveals dynamic assembly of the Wnt co-receptors LRP6 and ROR2 into common signalosomes.

Science signaling·2026

Same author

A dynamic displacement mechanism drives protein import into mitochondria.

bioRxiv : the preprint server for biology·2026

Same author

Disruption of ATP Synthase Spatiotemporal Organization, Ca<sup>2+</sup> Dynamics, and Contractile Function in Senescent Cardiomyocytes.

Aging cell·2026

Same journal

A Domino-Synthesized Dicoordinate Copper(I) Bis-imidazopyridine Complex Triggering Cuproptosis/Ferroptosis for Enhanced Cancer Immunotherapy.

Angewandte Chemie (International ed. in English)·2026

Same journal

Mirror-Symmetric Organic Two-Dimensional Crystals for Alternative Photon Transport Pathways.

Angewandte Chemie (International ed. in English)·2026

Same journal

Cobalt-Catalyzed Migratory E-Selective Asymmetric Aza-Nozaki-Hiyama-Kishi Coupling.

Angewandte Chemie (International ed. in English)·2026

Same journal

Facile Synthesis of α,ω-Dihydroxy Telechelic Macromonomers From Ethylene and α-Olefins for Recyclable Alternating Block Copolymers.

Angewandte Chemie (International ed. in English)·2026

Same journal

Multi-Atom Sub-Nanometer Assemblies on Interpenetrating Multi-Chambered N/C Nanospheres.

Angewandte Chemie (International ed. in English)·2026

Same journal

A Synergistic C<sub>2+</sub> Alcohols/Olefins-Intermediated Pathway Boosts CO<sub>2</sub> Hydrogenation to Aromatics.

Angewandte Chemie (International ed. in English)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells.

Christoph Drees¹, Athira Naduviledathu Raj², Rainer Kurre³

¹Abteilung für Biophysik, Fachbereich Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, 49076, Osnabrück, Germany.

Angewandte Chemie (International Ed. in English)

|August 12, 2016

Summary

This summary is machine-generated.

Engineered upconversion nanoparticles (UCNPs) efficiently detect protein interactions in living cells using lanthanide resonance energy transfer (LRET). These nanoparticles offer enhanced brightness and specific targeting for biological applications.

Keywords:

LRET TOM complexes biosensors nanoparticles upconversion

More Related Videos

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Related Experiment Videos

Last Updated: Mar 16, 2026

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

Area of Science:

Nanotechnology
Biophysics
Cell Biology

Background:

Upconversion nanoparticles (UCNPs) convert near-infrared light to visible light, offering advantages over traditional microscopy techniques.
Lanthanide resonance energy transfer (LRET) is a powerful tool for studying molecular interactions within cells.

Purpose of the Study:

To engineer UCNPs as efficient LRET donors for intracellular applications.
To develop a method for specific targeting and detection of protein interactions in living cells using UCNPs.

Main Methods:

Engineering <50 nm UCNPs with optimized dopant concentrations and core-shell structures.
Developing a biocompatible surface coating with a nanobody for GFP recognition.
Utilizing LRET to detect interactions of GFP-tagged fusion proteins in the mitochondrial outer membrane.

Main Results:

Enhanced UCNP emission and sensitized acceptor fluorescence observed at higher excitation densities.
Successful specific targeting of UCNPs to GFP-tagged proteins in living cells.
Demonstrated detection of protein interactions via LRET in a complex biological environment.

Conclusions:

Optimized UCNPs serve as efficient LRET donors for intracellular studies.
Biocompatible UCNP surface functionalization enables specific targeting and molecular interaction detection.
This approach provides a sensitive method for investigating protein interactions in living cells.