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Direct electrochemical determination of glucose oxidase in biological samples
A Aubrée-Lecat1, C Hervagault, A Delacour
1Laboratoire de Technologie Enzymatique, U.A. No. 523 du CNRS, Université de Compiègne, France.
Abstract:
An electrochemical method for the quantitation of glucose oxidase in murine plasma and tissues has been developed. Instead of oxygen, this method uses benzoquinone as an artificial cosubstrate of glucose oxidase. The quantitative detection of the enzymatically produced hydroquinone by controlled-potential amperometry allows measurement of glucose oxidase concentrations in biological samples. The use of an internal standard corrects for all possible interfering effects. We demonstrated a 10-fold increase in sensitivity, as well as the ability to work in turbid media, in comparison to spectrophotometric methods.