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Updated: Mar 16, 2026
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
Dissecting Plant Chromosomes by the Use of Ionizing Radiation
Penny M A Kianian1, Katie L Liberatore2, Marisa E Miller3
1Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St. Paul, MN, 55108, USA. kiani002@umn.edu.
Abstract:
Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high-resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.
