Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 16, 2026

2D and 3D Chromosome Painting in Malaria Mosquitoes
09:57

2D and 3D Chromosome Painting in Malaria Mosquitoes

Published on: January 6, 2014

10.9K

The Chromosome Microdissection and Microcloning Technique.

Ying-Xin Zhang1,2, Chuan-Liang Deng3, Zan-Min Hu4

  • 1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China.

Methods in Molecular Biology (Clifton, N.J.)
|August 12, 2016
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>OsLAP3/OsSTRL2</i>, encoding a rice strictosidine synthase, is required for anther cuticle formation and pollen exine patterning in rice.

Frontiers in plant science·2025
Same author

SIRT3 Regulates Levels of Deacetylated SOD2 to Prevent Oxidative Stress and Mitochondrial Dysfunction During Oocyte Maturation in Pigs.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2023
Same author

Lineage-specific amplification and epigenetic regulation of LTR-retrotransposons contribute to the structure, evolution, and function of Fabaceae species.

BMC genomics·2023
Same author

Antisense oligonucleotides targeting basal forebrain ATXN2 enhances spatial memory and ameliorates sleep deprivation-induced fear memory impairment in mice.

Brain and behavior·2023
Same author

Impact of LTR-Retrotransposons on Genome Structure, Evolution, and Function in Curcurbitaceae Species.

International journal of molecular sciences·2022
Same author

[Black microplastics in the environment: Origin, transport and risk of tire wear particles].

Ying yong sheng tai xue bao = The journal of applied ecology·2022
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study presents a refined chromosome microdissection technique. It enables precise chromosome analysis, DNA library construction, and gene mapping for genetic research.

Area of Science:

  • Cytogenetics
  • Molecular Genetics
  • Genomics

Background:

  • Chromosome microdissection and microcloning are vital tools in genetic research.
  • These techniques facilitate the creation of detailed genetic maps and gene localization.

Purpose of the Study:

  • To describe a modified, efficient technique for chromosome microdissection.
  • To demonstrate the application of this method for chromosome painting and DNA library generation.

Main Methods:

  • Modified chromosome microdissection protocol.
  • Single chromosome isolation and manipulation.
  • Development of chromosome-specific DNA libraries.

Main Results:

  • Successful microdissection of individual chromosomes.
Keywords:
Chromosome microdissection and microcloningDegenerated oligonucleotide-primed-PCRFluorescence in situ hybridizationSingle-chromosome library

More Related Videos

Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

84.2K
Micromanipulation of Chromosomes in Insect Spermatocytes
05:45

Micromanipulation of Chromosomes in Insect Spermatocytes

Published on: October 22, 2018

9.1K

Related Experiment Videos

Last Updated: Mar 16, 2026

2D and 3D Chromosome Painting in Malaria Mosquitoes
09:57

2D and 3D Chromosome Painting in Malaria Mosquitoes

Published on: January 6, 2014

10.9K
Chromosome Preparation From Cultured Cells
07:42

Chromosome Preparation From Cultured Cells

Published on: January 28, 2014

84.2K
Micromanipulation of Chromosomes in Insect Spermatocytes
05:45

Micromanipulation of Chromosomes in Insect Spermatocytes

Published on: October 22, 2018

9.1K
  • Efficient generation of chromosome-specific DNA libraries.
  • Demonstration of chromosome painting capabilities.

    • Conclusions:

    • The modified technique enhances the efficiency of chromosome analysis.
    • This method is valuable for constructing high-density genetic maps and isolating specific genes.