Related Experiment Video
Updated: Mar 16, 2026
Methods for the Determination of Rates of Glucose and Fatty Acid Oxidation in the Isolated Working Rat Heart
Published on: September 28, 2016
Effects and mechanisms of curcumin on the hemodynamic variablesof isolated perfused rat hearts
Erkan Kilinç1, Ziya Kaygisiz2, Bedri Selim Benek1
1Department of Physiology, Faculty of Medicine, Abant İzzet Baysal University, Bolu, Turkey.
Insights
Curcumin affects heart function in isolated rat hearts, decreasing heart rate and developed pressure while increasing end-diastolic pressure in a dose-dependent manner. Atropine reversed these hemodynamic effects.
Area of Science:
- Cardiovascular Physiology
- Pharmacology
Background:
- Limited data exists on curcumin's dose-response effects on cardiac hemodynamics at the organ level.
- Investigating curcumin's impact on isolated perfused rat hearts is crucial for understanding its cardiovascular mechanisms.
Purpose of the Study:
- To determine the dose-dependent effects of curcumin on hemodynamic variables in isolated perfused rat hearts.
- To elucidate the mechanisms underlying curcumin's cardiovascular actions.
Main Methods:
- Isolated rat hearts were perfused using a modified Krebs-Henseleit solution.
- Rats were divided into groups receiving saline, dimethyl sulfoxide, varying curcumin doses (0.2, 1, 5 µM), atropine, L-NAME, or combinations thereof.
- Hemodynamic parameters including heart rate and pressures were measured post-treatment.
Main Results:
- Curcumin (1 µM) significantly decreased heart rate and increased end-diastolic pressure.
- Curcumin (5 µM) reduced developed pressure and elevated end-diastolic pressure.
- Atropine administration counteracted the observed effects of curcumin on cardiac function.
Conclusions:
- Curcumin exhibits dose-dependent negative chronotropic and inotropic effects on isolated rat hearts.
- These findings suggest a direct impact of curcumin on cardiac contractility and rate regulation.
Background/Aim:
There is no information on the dose-response relationship of curcumin on the hemodynamic variables of the heart at the organ level in isolated perfused rat hearts. We aimed to investigate the effects and mechanisms of curcumin on the hemodynamic variables of isolated perfused rat hearts.
Materials And Methods:
Rats were randomly divided into 9 groups. The isolated rat heart was retrogradely perfused with modified Krebs-Henseleit solution. After the stabilization period, each group was administered one of the following treatments for 25 min: saline, dimethyl sulfoxide, and curcumin (0.2 µM, 1 µM, and 5 µM); atropine (1 µM); atropine (1 µM) + curcumin (1 µM); L-NAME (100 µM); or L-NAME (100 µM) + curcumin (1 µM). Hemodynamic variables of the heart were measured.
Results:
Curcumin at dose of 1 µM decreased the heart rate (from 271 ± 11.1 to 200.4 ± 14.3 beats/min, P = 0.011) but increased end-diastolic pressure (from 7.0 ± 0.4 to 54.6 ± 7.9 mmHg, P = 0.0008). A dose of 5 µM curcumin caused a decrease in the developed pressure (from 87.58 ± 9.0 to 65.40 ± 7.0 mmHg, P = 0.047) but an increase in the end-diastolic pressure (from 6.8 ± 0.6 to 48.9 ± 7.7 mmHg, P = 0.005). Atropine (1 µM) reversed the effects of curcumin on the heart.
Conclusion:
Our results suggest that curcumin produces dose-dependent negative chronotropic and inotropic effects in isolated perfused rat hearts.
More Related Videos
07:38Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect
Published on: June 14, 2015
06:22Author Spotlight: Advancing Cardiovascular Research — Tailored Langendorff Perfusion Techniques for Improved Experimental Outcomes
Published on: June 14, 2024
