Related Experiment Video
Updated: Mar 16, 2026
Generation of Self-assembled Vascularized Human Skin Equivalents
Published on: February 12, 2021
Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis
Claudia Pouliot-Bérubé1,2, Karine Zaniolo3, Sylvain L Guérin3,4
1Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada.
Aim:
Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used.
Materials & Methods:
Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray.
Results:
Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05).
Conclusion:
Psoriatic substitutes supplemented with a cocktail of TNF-α, IL-1α, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.
