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Updated: Mar 16, 2026

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
The effects of exercise modality on maximal and submaximal exercise parameters obtained by graded maximal exercise
Nils Cornelis1, Roselien Buys2
1Department of Rehabilitation Sciences, University of Leuven, Belgium.
Maximal cardiopulmonary exercise testing (CPET) on a supine bicycle (SB) yields lower peak oxygen uptake and heart rate compared to treadmill (TM) and upright bicycle (UB) tests. SB CPET results are not comparable to TM and UB for assessing maximal exercise capacity.
Area of Science:
- Cardiology
- Exercise Physiology
- Diagnostic Testing
Background:
- Cardiopulmonary exercise testing (CPET) is crucial for cardiac evaluation.
- Treadmill (TM) and upright bicycle (UB) are common CPET modalities.
- Supine bicycle (SB) testing is increasingly used, particularly with cardiac imaging.
Purpose of the Study:
- To compare maximal CPET results between SB, TM, and UB.
- To assess if peak oxygen uptake (VO2) on SB aligns with TM and UB.
- To investigate the impact of user preference and experience on peak VO2.
Main Methods:
- A randomized controlled cross-over trial involving 12 healthy individuals.
- Participants underwent maximal CPET on TM, UB, and SB.
- Statistical analysis included repeated measures ANOVA, ICC, and Bland-Altman plots.
Main Results:
- SB CPET showed significantly lower peak VO2 and peak heart rate than TM and UB.
- The VE/VCO2-slope did not differ significantly across the modalities.
- Low agreement was observed for SB measures, with no effect of preference or experience on peak VO2.
Conclusions:
- Maximal SB exercise testing is not equivalent to TM and UB for evaluating maximal exercise capacity.
- Current SB CPET protocols may not be suitable for direct comparison with established TM and UB benchmarks in clinical settings.
- Further research may be needed to validate SB CPET for specific clinical applications.
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