Related Experiment Video
Updated: Mar 16, 2026
06:48
On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
Published on: May 31, 2020
6.4K
Validating a robust double-quantum-filtered (1) H MRS lactate measurement method in high-grade brain tumours
G S Payne1, L M Harris2, G S Cairns2
1MRI Unit, Royal Marsden Hospital, Sutton, Surrey, UK. gsp@icr.ac.uk.
NMR in Biomedicine
|August 12, 2016
Summary
Double-quantum (DQ) filtering effectively removes confounding lipid signals for accurate lactate measurement using proton magnetic resonance spectroscopy (¹H MRS). This validated method is crucial for clinical applications, especially in lipid-rich tissues.
Area of Science:
- Biomedical Imaging
- Magnetic Resonance Spectroscopy
- Neuro-oncology
Background:
- Proton magnetic resonance spectroscopy (¹H MRS) measurements of lactate are frequently hindered by overlapping lipid signals.
- Lipid signals can obscure or mimic lactate peaks, leading to inaccurate quantification in clinical ¹H MRS.
- Existing methods often struggle with lipid interference, particularly in tissues with high lipid content or susceptibility to motion.
Purpose of the Study:
- To evaluate a single-voxel-localized double-quantum (DQ) filtered ¹H MRS method for measuring lactate.
- To assess the method's ability to eliminate lipid signals and quantify lactate in the presence of lipids.
- To compare DQ-filtered ¹H MRS with standard acquisition in high-grade brain tumors (glioblastoma).
Main Methods:
- Proton magnetic resonance spectroscopy (¹H MRS) was performed at 3 Tesla on patients with glioblastoma.
- Single-voxel localization using frequency offset corrected inversion pulses (fLASER) was employed for both standard and DQ-filtered acquisitions.
- Paired spectra were acquired and analyzed to compare lactate quantification and lipid signal suppression between the two methods.
Main Results:
- DQ-filtered lactate signals (1.33 ± 0.03 ppm) were distinct from lipid signals (1.22 ± 0.08 ppm), confirming lipid elimination.
- In 7 of 20 cases with measurable lactate, DQ filtering enabled quantification where standard acquisition failed due to lipid overlap.
- Lactate concentrations measured by DQ filtering and standard acquisition showed good agreement (mean ratio 1.10 ± 0.21) in the absence of significant lipid interference.
Conclusions:
- Single-voxel DQ filtering is a robust technique for eliminating lipid signals in ¹H MRS.
- This method allows for uncontaminated and accurate lactate quantification, even in the presence of confounding lipid signals.
- The validated DQ filtering technique holds significant promise for lactate measurements in lipid-rich tissues outside the brain.
