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Ivermectin exhibits potent anti-mitotic activity
Shoaib Ashraf1, Roger Prichard1
1Institute of Parasitology, McGill University-Macdonald Campus, Sainte-Anne-de-Bellevue, H9X 3V9 Quebec, Canada.
Veterinary Parasitology
|August 13, 2016
Summary
Ivermectin (IVM) interacts with mammalian tubulin, stabilizing microtubules and inhibiting cell division. This anti-mitotic effect is reversible, suggesting potential therapeutic applications.
Area of Science:
- Pharmacology
- Cell Biology
- Biochemistry
Background:
- Ivermectin (IVM) is an established anti-parasitic drug.
- Its primary mechanism involves glutamate-gated chloride channels (GluCls).
- Recent findings suggest IVM also interacts with nematode tubulin.
Purpose of the Study:
- To investigate the interaction of Ivermectin (IVM) with mammalian tubulin.
- To determine the effects of IVM on mammalian microtubule dynamics and cell division.
- To explore the potential of IVM as an anti-mitotic agent.
Main Methods:
- Tubulin polymerization assays were conducted.
- HeLa cells were exposed to IVM and subjected to cold-induced depolymerization.
- Cell replication and division were monitored in vitro.
Main Results:
- IVM demonstrated direct interaction with mammalian tubulin.
- IVM increased mammalian tubulin polymerization.
- IVM stabilized microtubules against depolymerization and inhibited HeLa cell replication reversibly.
Conclusions:
- Mammalian microtubules bind and are stabilized by IVM at micromolar concentrations.
- IVM affects tubulin polymerization/depolymerization dynamics, leading to cell death.
- IVM's anti-mitotic properties warrant further investigation for therapeutic development.
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