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Updated: Mar 16, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody
Published on: January 24, 2017
Production and Purification of Polyclonal Antibodies
Masami Nakazawa1, Mari Mukumoto2, Kazutaka Miyatake2
1Department of Applied Biological Chemistry, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan. mami@biochem.osakafu-u.ac.jp.
Abstract:
Polyclonal antibodies consist of a mixture of antibodies produced by multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against an antigen recognize multiple epitopes on a target molecule, which results in a signal amplification in indirect immunoassays including immune-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
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