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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.7K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Related Experiment Video

Updated: Mar 16, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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Pre-embedding Nanogold Silver and Gold Intensification.

Akitsugu Yamamoto1, Ryuichi Masaki2

  • 1Department of Animal Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga, 526-0829, Japan. a_yamamoto@nagahama-i-bio.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2016
PubMed
Summary

Pre-embedding nanogold intensification methods offer high sensitivity and resolution for immunolabeling. Silver intensification excels in labeling, while gold intensification provides superior ultrastructural preservation in electron microscopy.

Keywords:
Cultured cellsGold intensificationNanogoldPre-embedding immunoelectron microscopySilver intensification

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Immunohistochemistry

Background:

  • Pre-embedding immunolabeling is crucial for visualizing cellular structures.
  • Nanogold and intensification methods enhance sensitivity and resolution in electron microscopy.
  • Simultaneous light and electron microscopy is valuable for correlative studies.

Purpose of the Study:

  • To introduce pre-embedding nanogold silver and gold intensification methods.
  • To detail procedures for application primarily with cultured cells.
  • To compare the efficacy of silver and gold intensification.

Main Methods:

  • Immunoreactions using nanogold-labeled antibodies.
  • Pre-embedding intensification of nanogold particles.
  • Ultrathin sectioning for electron microscopy.
  • Application to cultured cells.

Main Results:

  • High sensitivity and good resolution achieved with both methods.
  • Silver intensification generally superior for immunolabeling intensity.
  • Gold intensification offers better ultrastructural preservation.
  • Methods enable simultaneous light and electron microscopy observation.

Conclusions:

  • Pre-embedding nanogold silver and gold intensification are valuable techniques.
  • Choice between silver and gold depends on whether labeling or ultrastructure is prioritized.
  • These methods facilitate advanced imaging, including 3D immunoelectron microscopy.