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Identification and partial separation of three distinct 32-kDa calcium/phospholipid-regulated proteins from bovine
1Department of Biochemistry, Akita University School of Medicine, Japan.
Abstract:
We have separated three distinct 32-kDa calcium/phospholipid-regulated proteins from bovine spleen, which we have designated as 32kDa-Ia, 32kDa-Ib and 32kDa-II. By one-dimensional peptide mapping and chromatographic behavior, the three proteins are distinct from each other. Gizzard 35kDa calcimedin antibody recognizes both 32kDa-Ia and 32kDa-II but not 32kDa-Ib. Anti-aorta endonexin II specifically reacts with 32kDa-II. Bovine lens endonexin antibody reacts with 32kDa-Ia and 32kDa-Ib, but not with 32kDa-II. These data suggest that the 32-kDa calcium/phospholipid-regulated proteins purified from various sources can be divided into three distinct classes of proteins.