Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A nuclear casein type II kinase from maize endosperm phosphorylating HMG proteins.

K D Grasser1, U G Maier, G Feix

  • 1Institute for Biology III, Albert-Ludwigs-Universität, Freiburg, FRG.

Biochemical and Biophysical Research Communications
|July 14, 1989
PubMed
Summary

A novel casein kinase II was identified in maize endosperm, showing specific activity towards high mobility group (HMG) proteins. This enzyme requires magnesium and can use ATP or GTP, but is inhibited by polyamines and heparin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Review: origin of complex algae by secondary endosymbiosis: a journey through time.

Protoplasma·2017
Same author

Transcriptional effects of the opaque-2 mutation of Zea mays L.

Planta·2013
Same author

Characterization of a maize endosperm culture expressing zein genes and its use in transient transformation assays.

Plant cell reports·2013
Same author

Isolation and characterization of high-mobility-group proteins from maize.

Planta·2013
Same author

Early post-embryonic root formation is specifically affected in the maize mutant lrt1.

The Plant journal : for cell and molecular biology·2012
Same author

Focus broadening by astigmatism of large microwave parabolic antennas.

Applied optics·2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Plant Science

Background:

  • Nuclear protein phosphorylation plays a crucial role in gene regulation and cellular processes.
  • Casein kinases are important enzymes involved in various cellular signaling pathways.
  • Understanding plant-specific kinases is essential for deciphering gene expression in crops like maize.

Purpose of the Study:

  • To isolate and characterize a casein kinase from maize endosperm nuclear lysates.
  • To determine the enzymatic properties and substrate specificity of the identified kinase.
  • To investigate the potential role of this kinase in maize endosperm nuclear protein regulation.

Main Methods:

  • Isolation and enrichment of casein kinase activity from maize endosperm nuclear fractions.

Related Experiment Videos

  • Enzymatic assays to determine optimal conditions (Mg2+ concentration, phosphate donors).
  • In vitro phosphorylation assays using purified high mobility group (HMG) proteins and histones as substrates.

    • Main Results:

    • A casein kinase of type II was successfully isolated and enriched from maize endosperm nuclear lysates.
    • Maximal kinase activity was observed at 10 mM Mg2+, utilizing ATP or GTP as phosphate donors.
    • The kinase exhibited substrate specificity, phosphorylating HMG proteins but not histones.

    Conclusions:

    • Maize endosperm nuclear lysates contain a casein kinase II with specific activity towards HMG proteins.
    • This kinase's properties suggest a role in regulating nuclear protein function within maize endosperm.
    • Further studies are warranted to elucidate the precise biological function of this HMG-protein-specific kinase in maize.