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Adsorptive stripping voltammetry of human chorionic gonadotropin and two of its specific antibodies
J Rodriguez Flores1, R O'Kennedy, M R Smyth
1Department of Analytical Chemistry and Electrochemistry, Universidad de Extremadura, Badajoz, Spain.
Biosensors
|January 1, 1989
Abstract:
The electrochemical behaviour of human chorionic gonadotropin (hCG), as well as rat anti-beta hCG and rabbit anti-beta hCG antibodies, has been studied using cyclic voltammetry and adsorptive stripping voltammetry. Conditions have been optimised for the determination of these species by differential pulse adsorptive stripping voltammetry with respect to accumulation potential, accumulation time, scan rate, pulse amplitude and drop size. This technique has also been used to investigate the interaction of hCG with each of its specific antibodies in solution.